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Pdgfb-creert2

SpletThe targeting vector used to modify PAC 546-M3 is illustrated (a). The construct was designed to insert the tamoxifen-inducible form of Cre recombinase (CreERT2) (Indra et al., 1999) into the first coding exon of the Pdgfra gene (exon 2). Homology regions 0.5 kb in length were amplified by PCR from the genomic PAC using Expand High Fidelity ... Splet01. apr. 2016 · To test this proposed mechanism in adipose tissue, we deleted Flt1 in endothelial cells of Flt1-fl/fl;Pdgfb-CreERT2 (Flt1-EC KO) mice by tamoxifen administration (Figures S10A–S10D). This resulted in reduced levels of VEGFR1, with the remaining protein in adipose tissue and serum likely produced by non-endothelial cells such as macrophages.

PDGFB - an overview ScienceDirect Topics

SpletFlt1fl/fl;Pdgfb-CreERT2 mice induced a significant reduction in VEGFR1 (a) mRNA and (b) protein levels in adipose tissue and (c) soluble VEGFR1 protein concentration in serum. (d) Recombination induction by tamoxifen-activated Pdgfb-CreERT2 in endothelial cells of adipose tissue was validated in STOP-lox Rosa26-TdTomato reporter ... Splet一、品系介绍： Cre-ERT2小鼠是一类含有雌激素受体（estrogen receptor，ER）的配体结合区突变体（ERT）与Cre重组酶的融合蛋白表达的小鼠。 Cre-ERT2在无Tamoxifen诱导的 … maes y ffynnon aberdare https://clustersf.com

PDGFRβ-P2A-CreERT2 mice: a genetic tool to target pericytes in ...

Splet16. nov. 2024 · In the PdgfbCreERT2; R26R-EYFP reporter line, the endocardium (arrowheads, Q) and all endothelial cells of subendocardial vessels formed after MI were entirely negative for EYFP (P-R), confirming... Splet06. jan. 2016 · Pdgfb-creERT2-induced overexpression of MYC caused sustained vascular overgrowth and led to a profound increase in EC number, proliferation and vessel density … Splet25. okt. 2024 · The Pdgfb retention motif knockout mouse (Pdgfb ret/ret) is a hypomorphic Pdgfb model that has been used to study postnatal effects of pericyte loss, particularly in … maes y pandy llanuwchllyn

The Cre-recombinase is expressed in hematopoietic cells in ...

Category:Revisiting PI3-kinase signalling in angiogenesis - PMC

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Pdgfb-creert2

Endothelial-Specific Cre Mouse Models Arteriosclerosis

SpletCre-mediated recombination occurs in a small number of adult hematopoietic stem cells in Pdgfb-iCreERT2;JAK2 V617F/WT transgenic mice. SUMMARY: Background The vessel … Splet29. nov. 2024 · Pdgfb-CreERT2, Pten Flox/Flox: Endothelium: Vascular hyperplasia in a retina model as a result of uncontrolled stalk cell proliferation (16) AKT: Akt1−/−: Global: Viable, defective ischemia-induced angiogenesis and endothelial progenitor cells recruitment (19, 21) Cdh5-CreERT2, Akt1Flox/Flox: Endothelium

Pdgfb-creert2

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SpletThe Tg (Pdgfb-icre/ERT2) 1Frut allele was created by insertion of Cre/ERT2 coding cDNA at the start codon of the Pdgfb gene in a PAC, which was … Splet02. maj 2024 · To further explore the need of Casp8 in lymphatic and blood ECs, we crossed the PDGFb-CreERT2 mouse line (shown to specifically recombine in blood ECs (BECs) in the intestine (Bernier-Latmani et al, 2015); here also confirmed by GFP expression in CD31 + /Lyve-1-cells (Appendix Fig S3A)) with Casp8 fl/fl mice.

Splet20. jan. 2024 · The Pdgfb-CreER strain was crossed with a Nrp1-floxed strain to produce offspring with or without the CreER transgene in a homozygous Nrp1-floxed background. Upon tamoxifen treatment, Nrp1 protein was depleted in choroidal endothelial cells (arrows in Fig 1) in CreER positive (Nrp1 ΔEC) but not in CreER negative (Wt) animals ( Fig 1G–1J ). SpletAltogether the results obtained with mT/mG reporter mice suggest, as previously published, that Cre is not expressed in the hematopoietic lineage of adult inducible Cdh5 (PAC)-CreERT2 and Pdgfb ...

Splet13. apr. 2024 · Platelet-derived growth factor (PDGF) signaling is frequently aberrantly activated in GBM, primarily due to the amplification of the PDGF-receptor alpha ( PDGFRA) 25, 26 (Supplementary Fig. 2a ). SpletFumiaki Uchiumi, in Bidirectional Gene Promoters, 2024. Growth factors and receptors. The PDGFB (c-sis) [12] and CSF1R (c-fms) [13] are the earliest identified proto-oncogenes, …

Splet10. jan. 2013 · In this review, we described several Cre lines that target ECs, including Tie2-Cre, VE-Cadherin-CreERT2, PDGFB-CreERT2, and others. Many of these Cre alleles target all ECs, and therefore it would be helpful to generate Cre alleles and CreERT alleles that specifically target CNS ECs. To do so, one could take advantage of the split-Cre system.

Splet19. feb. 2024 · We engineered these viruses to coexpress CreERT2 with PDGFB, allowing for deletion of floxed genes specifically in transduced cells, and designed another version … maes y garth b\\u0026bSplet01. feb. 2008 · Pdgfb-iCreER T2 is a widely used transgenic mouse line that expresses a tamoxifen-inducible form of Cre-recombinase (iCreER T2 ) 16 in endothelial cells. Pdgfb-iCreER T216 mice were crossed... maes y mynydd term datesSplet07. mar. 2024 · Tg (Pdgfb-icre/ERT2,-EGFP)1Frut. Mutation details : The mouse Pdgfb promoter was used to drive inducible expression of an improved cre gene fused to ERT2 … maes y rhedyn talbot greenSplet16. nov. 2024 · Pdgfb CreERT2; R26R-EYFP mice were injected with 2 doses of 2 mg tamoxifen to prelabel coronary endothelial cells, 2 weeks prior to myocardial infarction ( A ), and assessed before/after infarction by immunostaining. maes y glyn aberffrwdSpletWe observed that Pdgfb-iCreERT2 and Cdh5(PAC)-CreERT2- mediated recombination occurs in the vast majority of blood endothelial cells in the brain, retina, lungs, kidney and … maes y rhedyn maerdySplet029684 - Pdgfr-β-CreER [T2] Strain Details. Technical Information Scientists. Overview. maes y rhedynSplet28. apr. 2024 · d, Induction of Krit1 deletion and deletion of either one or both alleles of Pten in neonatal endothelial cells using a Pdgfb–CreERT2 transgene with brains harvested at … kitchen vanity with sink home depot