Pin1 activity assay kit

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WebAug 2, 2024 · We expressed A. thaliana PIN1 in HEK293F cells (Extended Data Fig. 2a) and characterized its transport activity. In the loading assay, cells co-expressing PIN1 and D6PK accumulated much less ... WebJun 2, 2014 · Specific activity is > 330 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay. 1. Prepare 180ul assay buffer into a suitable container: 133mM Tris-HCl, pH8.0, 5.5nM chymotrypsin, and 0.5ug of Pin1 recombinant protein.

Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced …

WebApr 14, 2024 · The protein concentrations of whole-cell lysates were measured by the Pierce BCA Protein Assay Kit (23225). Equal amount of protein lysates was resolved by SDS-PAGE gels and then transferred on a PVDF membrane (Millipore). ... Taken together, our results suggest that Pin1 facilitates AKT activity to promote SIK1 ubiquitination and … WebSep 23, 2024 · KPT-6566 covalently binds to PIN1 PPIase, and inhibits the PPIase activity of PIN1, with an IC50 of 0.64 μM. It selectively inhibits PIN1 instead of other PPIases. KPT-6566 does not affect the PPIase activity of recombinant GST-FKBP4 and GST-PPIA, which also own cysteine residues. In a further study, it proves that KPT-6566 affects PIN1 ... is hexavalent chromium banned

SensoLyte® Green PIN1 Activity Assay Kit Fluorimetric - 1 kit

WebJun 25, 2024 · Cell Calcium Assay Kit. Calcium concentrations were measured using a biochemical testing kit for cell calcium assay (Genmed, Shanghai, China) according to the manufacturer’s instructions. ... These results suggest that the Pin1 activity might be affected by KN, but not MC and BA, in this glutamate model. WebSensoLyte® Green Pin1 Assay Kit (Fluorimetric), AnaSpec Inc. Supplier: AnaSpec Peptidyl-prolyl isomerase (PPIase) Pin1 catalyzes cis/trans isomerization of the phospho-Serine/Threonine-Proline peptide bonds The SensoLyte® Green Pin1 Assay Kit uses a … WebNote 3: The amount of Pin1 enzyme is enough for 20 assays, the amounts supplied of the rest of the components are sufficient to run 100 assays. More Pin1 enzyme can be purchased at Abcam (Cat.#51230). Note 4: Do not re-freeze and re-use Pin1 Developer … sabp crisis number

An irreversible inhibitor of peptidyl-prolyl cis/trans isomerase Pin1 ...

SensoLyte Green Pin1 Activity Assay Kit Fluorimetric*

WebTumor suppressive activity of prolyl isomerase Pin1 in renal cell carcinoma. Mol Oncol.. 2011-10; 5 (5):465-74. Teng BL, Hacker KE, Chen S, Means AR, Rathmell WK. a Department of Pharmacology and Cancer Biology, Duke University, 308 Research Drive, Durham, NC 27710, USAb Lineberger Comprehensive Cancer Center, University of … WebFeb 1, 2024 · Pin1 (protein interacting with never in mitosis A-1) is a member of the peptidyl prolyl isomerase (PPIase) family, and catalyzes cis-trans isomerization of pThr/Ser-Pro amide bonds. ... This compound inhibited Pin1 in protease-coupled assay, and formed a covalent bond with Cys113 of Pin1, as determined by ESI-MS. The acetoxymethyl ester … is hexaware a good company for freshersWebApr 27, 2015 · Pin1 (Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl isomerase, PPIase) is an enzyme that isomerizes phospho/Threonine-Proline motifs. Upon binding (to various target proteins) it can function as a protein regulator as it plays a role … sabp twitter

"WebThe ELISA kits listed below target PIN1, the symbol for the human gene, peptidylprolyl cis/trans isomerase, NIMA-interacting 1. The protein has an enzyme classification of Peptidylprolyl isomerase. The protein encoded by PIN1 has a predicted amino acid … " - Pin1 activity assay kit

Pin1 activity assay kit

First Pin1 Alzheimer research assay on the market

WebDec 1, 2015 · Knockdown of Pin1 inhibited alcohol-induced cardiomyocyte apoptosis. (a) Pin1 protein expression measured by western blotting after Pin1 siRNA transfection. ((b)–(d)) Cell viability assay (b), caspase-9 and caspase-3 activity assays (c), and TUNEL staining (d) in Pin1-knockdown cardiomyocytes treated or untreated with alcohol (200 … WebHuman Pin1 ELISA. RayBio® Human Pin1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants. Lead time: Typically ships within 1-2 business days. No Friday shipments. $480.00. CODE: ELH-PIN1-1. Number of Plates: Quantity: + −.

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Web第一部分、Pin1基因敲减对SH-SY5Y神经细胞氧反常损伤的影响目的：观察Pin1在不同时限SH-SY5Y神经细胞氧反常损伤中的表达变化，通过Pin1基因敲减，研究Pin1对细胞凋亡的影响及在线粒体凋亡通路中的作用机制，探讨Pin1在SH-SY5Y神经细胞氧反常损伤中的功能。方法：（1）Pin1蛋白的细胞内定位 ... WebPin1 Activity Assays. Pin1 activity was measured as previously described , with a few modifications. Briefly, for analysis of Pin1 inhibitors, SN were pretreated for 15 min at 37°C with drugs before lysates were prepared by addition of Triton X-100 to a final concentration of 1%, followed by three freeze-thaw cycles and 20 min incubation on ice.

WebJun 2, 2014 · Specific activity is > 330 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay. 1. Prepare 180ul assay buffer into a suitable container: … WebThis high-throughput assay system is a powerful tool for the discovery of peptidyl- ... ning time to monitor PPIase activity on one 96-well microplate is about 30 min,10 which is problematic for high-throughput screening (HTS). ... kit, HBS-EP buffer, and anti-GST antibody were obtained from GE Healthcare Bio-Sciences (Tokyo, Japan). ...

WebProtease inducers and inhibitors are crucial targets for the development of therapeutics and other anti-cancer strategies. AnaSpec’s SensoLyte® Cancer and Apoptosis protease assay kits are ideal for High Throughput Screening in continuous assay of proteases activity, protease inhibitors and inducers using proprietary substrates. WebThe SensoLyte® Green Pin1 Assay Kit uses fluorogenic substrate that was pretreated to convert into cis isoform. Pin1 changes substrate into trans conformation that is readily cleaved to generate fluorescent signal which can be monitored at Ex/Em=490/520nm. Increase in fluorescence intensity is directly proportional to the Pin1 activity. Order #:

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WebSep 17, 2024 · The inhibitory activities of our Pin1 inhibitors, H-77, H-175, H-363, H-371 and H-596, have been confirmed to inhibit Pin1 enzyme activity in an in vitro PPIase assay using recombinant Pin1 ... is hexavalent chromium the same as chromium 6WebPin1 is a PPIase specific for pSer/pThr-Pro motifs; Uses recombinant human Pin 1 and substrate Succ-AEPF-pNA; Measures cis/trans isomerisation kinetics of substrate using UV/Vis spectrophotometer; Uses LiCl / Trifluoroethanol. Li+ ion holds substrate in 60% … is hexavalent chromium the same as chromiumWebNov 28, 2014 · Luciferase activity was determined using a luciferase assay kit (Promega) according to the manufacturer’s instructions. The relative luciferase activity was normalized against the β-galactosidase activity. ... To further understand how Pin1 is down-regulated by p53 during ER stress, we examined the effect of p53 on Pin1 promoter activity. sabp mental health teamWebThe ELISA kits listed below target PIN1, the symbol for the human gene, peptidylprolyl cis/trans isomerase, NIMA-interacting 1. The protein has an enzyme classification of Peptidylprolyl isomerase. The protein encoded by PIN1 has a predicted amino acid length of 163, a mass of 18.2 kDa, and a cytoplasmic and nuclear subcellular localization. is hexavalent chromium radioactiveWebApr 8, 2024 · Liu et al. reported that an IRAK1-PIN1 axis enhances intrinsic tumor resistance to radiation therapy in p53 ... DNA damage was detected with a comet assay kit (KeyGEN, Nanjing, China) under alkaline conditions. ... s Luciferase reporter assay for detecting the activity of wild-type or mutant IRAK1 promoters in U251 cells which were transfected ... is hexaware a listed companyWebFeb 13, 2024 · SensoLyte® Green PIN1 Activity Assay Kit Fluorimetric - 1 kit $607.00 Check your price Cat.Number : AS-72240 Availability : In … is hexclad teflonWebPIN1 is expressed in the protoderm, its polarity facing the cotyledon initiation sites, and in the vasculature, where polarity is facing downwards ( Benkova et al., 2003 ). The cotyledon initiation sites are marked by convergence of PIN1 in the adjacent membranes of … sabp nhs foundation trust